Layer-specific changes in sensory cortex across the lifespan in mice and humans

The segregation of processes into cortical layers is a convergent feature in animal evolution. However, how changes in the cortical layer architecture interact with sensory system function and dysfunction remains unclear. Here we conducted functional and structural layer-specific in vivo 7T magnetic resonance imaging of the primary somatosensory cortex in two cohorts of healthy younger and older adults. Input layer IV is enlarged and more myelinated in older adults and is associated with extended sensory input signals. Age-related cortical thinning is driven by deep layers and accompanied by increased myelination, but there is no clear evidence for reduced inhibition. Calcium imaging and histology in younger and older mice revealed increased sensory-evoked neuronal activity accompanied by increased parvalbumin expression as a potential inhibitory balance, with dynamic changes in layer-specific myelination across age groups. Using multimodal imaging, we demonstrate that middle and deep layers show specific sensitivity to aging across species.

Recommended citation: Liu, P., Doehler, J., Henschke, J. U., Northall, A., Knaf-Serian, A., Loaiza-Carvajal, L. C., Budinger, E., Schwarzkopf, D. S., Speck, O., Pakan, J. M. P., & Kuehn, E. (2025). Layer-specific changes in sensory cortex across the lifespan in mice and humans. Nature Neuroscience, 28（9），1978-1989.
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